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Detection of Bacillus cereus isolated during ultra high temperature milk production flowchart through random amplified polymorphic DNA polymerase chain reaction Ciência Rural
Vidal,Ana Maria Centola; Rossi Junior,Oswaldo Durival; Abreu,Irlan Leite de; Bürger,Karina Paes; Cardoso,Marita Vedovelli; Gonçalves,Ana Carolina Siqueira; Rossi,Gabriel Augusto Marques; D'Abreu,Léa Furlan.
ABSTRACT: The present study focused on isolation Bacillus cereus during the UHT milk production and shelf life, to assess the enterotoxigenic production capacity of isolates and to evaluate the use of the RAPD-PCR technique to verify whether Bacillus cereus isolated at different phases of UHT milk processing belongs to the same strain. For this, six groups of milk samples composed of raw, pasteurized and UHT milk were collected from a processing plant. The results revealed that bacteria belonging to the Bacillus cereus group were isolated from 51.6%, 81.6% and from 13.8% of raw, pasteurized and UHT milk samples, respectively. About 50.0% of isolates from raw milk, 19.2% isolates from pasteurized milk and 70.7% isolates from UHT milk were capable of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus cereus; Enterotoxins; Microbiological quality; Pasteurized milk; Raw milk; UHT milk.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000200286
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Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis Ciência Rural
Gonçalves,Ana Carolina Siqueira; Roma Júnior,Luiz Carlos; Privatti,Rafaela Torrezan; Salles,Márcia Saladini Vieira; Paz,Claudia Cristina Paro de; Zadra,Lenira El Faro; Vidal,Ana Maria Centola.
ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monitoring; Udder health; Alternative method; Somatic cell count.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600454
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